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Roopa, G.
- ITS Sequencing of Indian Isolates of Lecanicillium Species
Abstract Views :213 |
PDF Views:179
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 337-341Abstract
The genetic diversity of thirty one isolates of Lecanicillium species isolated from insect hosts from various geographical regions of India were studied. Their phylogenetic relationships were determined using internal transcribed spacer, ITS1, ITS2 and 5.8S gene of rRNA sequence. Based on the sequence similarity of ITS region and construction of subsequent phylogenetic analysis (neighbour-joining method), 15 isolates were grouped as Lecanicillium lecanii, 11 isolates as L. attenuatum, 3 isolates as L. longisporum and 2 isolates as L. muscarium.Keywords
ITS Region, Phylogenetic Analysis, Lecanicillium sp.Full Text
References
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- Zare, R. and Gams, W. 2001. A revision of Verticillium section Prostrata. IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia, 73: 1–50.
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- Chitinase Activity and Virulence of Different Isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium Spp
Abstract Views :287 |
PDF Views:129
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 3 (2011), Pagination: 223-228Abstract
In order to identify promising isolates with higher chitinase activity, 122 entomofungal isolates comprising of Beauveria bassiana (58), Metarhizium anisopliae (33), Lecanicillium lecanii (15), Lecanicillium attenuatum (11), L. longisporum (3) and L. muscarium (2) isolated from different insect hosts/soils of India were studied. The partially purified proteins from the isolates were subjected to chitinase activity and were estimated by measuring the release of reducing saccharides (one NAGA Unit) from colloidal chitin spectrophotometrically at 582 nm (A582). Forty nine isolates of B. bassiana showed chitinase activity ranging from 21 to 182 μg/ml, with the highest enzyme activity by the isolate PDBC-Bb-5a. Thirty three isolates of M. anisopliae exhibited chitinase activity ranging from 23 to 144 μg/ml and the highest (144 μg/ml) was by the isolate Ma-4. Among the 15 isolates of L. lecanii tested, three isolates viz., Vl-7, Vl-24a, Vl-25a had high chitinase activities ranging between 100 and 126μg/ml. Vl-22 isolate of L. attenuatum, Vl-24 of L. longisporum and Vl-8 of L. muscarium showed higher activities (90, 110 and 117μg/ml respectively). Bioassay studies with these isolates on cowpea aphid, Aphis craccivora in glass house indicated higher nymphal mycosis ranging from 72.3-83.0%.Keywords
Entomopathogenic Fungi, Chitinase Activity, NAGA Unit, Cowpea Aphid, Aphis craccivora, Virulence.Full Text
References
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